From the same site, a second aspiration or an aspirate of > 10 mL can be drawn without any loss of biological quality due to dilution with peripheral blood.
Results There was a high correlation between the first and second aspiration volumes, and aspirates with a volume of less than 8 mL showed a large variation in cellular yield.
There was about half a minute between the first and second aspiration.
Membrane tethers were pulled with the help of a streptavidin-coated bead that was moved with a second aspiration pipette.
Where aspiration obtained notably less synovial fluid than expected from clinical examination (n = 5), a second aspiration was attempted using a medial approach.
On average, the second aspiration contained 33% less hMSCs than the first, which was not statistically significant (95%CI: -2.7 × 10 – 8.0 × 10).
Both basic and dex-induced ALP expression was lower in the second aspiration; however, no statistically significant differences were observed (95%CI: -0.37 15).
The beads were hereafter picked up by the Vacuum Prep Tool (Biotage AB, Uppsala, Sweden) and were rinsed by sequential 10-second aspirations of 70% ethanol, 0.2 M NaOH and 70% ethanol, followed by release into 15 μl deionised formamide (Amresco, Solon, OH, USA).
One way out was music: Coates's first aspiration was to be a rap artist.
Joyce said his first aspiration was "that the agreement remains confidential".
