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After the first immunization, the titers of anti-rOmpF against rOmpF were 1 100 1 300, and rised sharply to 1 27,000 1 240,000 after the second and third immunization (Fig. 4A).
Five BALB/c mice were immunized by intraperitoneal injection of the 200 μl suspension containing 108 inactivated bacteria per ml or OMPs mixed with an equal volume of Freund's adjuvant (complete Freund's adjuvant for the first immunization and incomplete for the second and third immunization) for three times at 2-week intervals.
Bleeding was performed 10 days after the second and third immunization.
While all volunteers in the 50 µg groups reached a titer >1∶500 after the second and third immunization, none of the PEV301 10 µg group reached this titer (Table 3).
ELISPOT responses to the CS antigen were significantly higher than pre-vaccination values after the second and third immunization in RTS,S/AS01E and RTS,S/AS02D vaccinated children.
This finding raises the question of whether an effective immunization regimen can be limited to two immunizations, or alternatively, how the time interval between second and third immunization could be optimized.
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One milliliter of peripheral blood was collected on day 0 (pre-immune or P0) and 20 days after the second and third immunizations (II20 and III20, respectively) to obtain lymphocytes and sera for immunological studies.
The vaccine and dose assigned during the first immunization were maintained for second and third immunizations.
An increase in frequency and intensity of systemic adverse events in the second and third immunizations as compared to the first was also noted.
This dramatic increase in anti-AMA1 antibody concentrations continued to be significant post second and third immunizations on Days 42 and 70 and to the end of the study on Day 236.
The malaria vaccine full-dose group had the most general solicited signs and symptoms during the 8-d post-immunization periods, with 14, 15 and 10 general adverse events following the first, second and third immunizations, respectively, compared to 18, 4 and 6 in the half-dose group and 2, 14 and 3 in the rabies vaccine group (Table 2).
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