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The impact of co-administration of vaccines on antibody avidity was calculated with a two-tailed unpaired t test comparing avidities after first, second, and third immunisation.
The identical injection sites were used for the first, second and third immunisation.
The birds immunised with H1, H9, H10 and H16 were given live virus orally and into the conjunctiva at the first immunisation and subsequently immunised with inactive allantoic fluid with incomplete Freunds adjuvant at the second and third immunisation.
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The second and third immunisations in this volunteer were well tolerated; induration did not re-appear.
To assess long-term immunological responses, the interval between the second and third immunisations was 17 weeks.
The score of nausea and vomiting item was significantly greater after the third and fourth immunisation.
Surprisingly, despite that fact that the family received a set of plates for the last immunisation, the biggest increase between intervention A and intervention B villages was for the third and fourth immunisation.
One (Alhydrogel™ 10 µg group) developed a generalised rash assessed as unrelated to the vaccine between the first and second immunisations and one (Montanide 10 µg group) received a concomitant hepatitis B immunisation.
Nevertheless, the presence of neutralising antibodies against BoHV-1 after the first and second immunisations suggests the replication of gE-negative BoHV-1 in goats.
The second and third group immunisations (Gp 2 and Gp 3) were done simultaneously in the same experiment at BioGenes, while the first and fourth (Gp 1 and Gp 4) were done in separate experiments.
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