The first and third copies are identical to each other, but the second copy differs slightly (5/3352 nucleotide differences).
The first and third copies of the 3.3-kb region have 3/3352 nucleotide differences with NA, Zmp, and CMS-S, and 12/3357 nucleotide differences with CMS-T.
These sequence comparisons indicate that the second 3.3-kb region is more similar to the NA, Zmp, and CMS-S mitochondrial genomes than the first and third copies of the 3.3-kb region.
† AAT27 specifies a tandem pair; AAP8LD and AAP11LD refer to the first and second copies respectively in the classification of Akerman et al. 2004.
Transposition of the first and second copies was required to account for AAT23Lm, which is a tandem pair that clustered within the AAT1 clade (as described above).
Using capillary sequencing, we identified the event as being a tandem duplication of 148 951 bp at chr20 9,684,767 9,833,717 hg18) with a 15 bp sequence inserted between the first and second copies of the repeated sequence (Fig. 2).
By capillary sequencing this individual, we identified a tandem duplication of 148 951 bp at chr20 9,684,767 9,833,717 hg18) with a 15 bp sequence inserted between the first and second copies of the repeated sequence (Fig. 2).
In the sulfonamide- and trimethoprim-resistant S. agalactiae isolate (21), expression of the second, third, and fourth copies of the fol genes was ascribed to an alternative promoter, but this led to only a 5-fold increase in gene expression, rather than the 4-fold increase that would be expected from gene dosage alone.
