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In case of the second 1 week exposure in 2010, CT was measured using an AIRICA CT analyzer (Marianda GmbH, Kiel, Germany).
RNA from whole left gills #9 (only day 7, control CO2, n = 6; elevated CO2, n = 6) from the second 1 week experiment (2010) and from whole left gills #7 (control CO2, n = 9; elevated CO2, n = 9) of the 11 week experiment was extracted using the RNeasy Midi-Kit from Qiagen (#75144).
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All patients recruited must consent to sampling at the second (15 week) and third (20 week) time-points.
Baseline data were collected the first 4 weeks; rounding was done the second 4 weeks.
Group A received WEP for the first 12 weeks, followed by no intervention for the second 12 weeks.
Group B did not receive intervention for the first 12 weeks and received WEP for the second 12 weeks.
WEAN steers gained faster during the first 2 weeks (P=0.02) and SHIP steers were more efficient during the second 2 weeks (P=0.03).
Intervention Subjects consumed a placebo twice daily with meals for the first 2 weeks (baseline) and PYC (200 mg/day) for the second 2 weeks.
Out of 7 CCH patients, six reported a progressive reduction of bouts in terms of number and intensity (three during the first 4-week period, one during the second 4-week period, one during the third 4-week period).
In order to stimulate multiple flowering inflorescences, the temperature and/or light spectrum during the second 4-week-period appears to be more important than during the first 4-week-period of the induction phase.
Two patients received a second 2-week course.
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