Sentence examples for sec water from inspiring English sources
Three sec after CS onset, the bee got its antennae and proboscis stimulated with its particular treatment during 6 sec (water, quinine, salicine, NaCl, or mechanosensory stimulation in the pre-exposure phase; sucrose 1 M in the conditioning phase; see above).
After removal of the samples every spot was washed twice using binding buffer followed by a final 10 sec water rinse.
Purified antibodies were analysed for purity by SDS-PAGE and Bioanalyzer 2000, aggregation by uplc-sec (Waters), concentration by Pierce BCA kit (ThermoFisher) and endotoxin by endosafe PTS (all <0.025 EU/mg, Charles River).
The maximum yield of hydrogen production was 7.967% under the conditions of microwave power of 500 W, exposure time of 120 sec, and water bath at 55°C.
Alkaline phosphatase with Kiel [19]: 2 min Kiel solution [6% ethylenglycol monoethylether (MERCK KGaA), 1 mM naphtol AS-BI phosphate (Sigma-Aldrich), 0.01% new fuchsin (VWR, Bie & Berntsen), 0.02% NaNO2 (VWR, Bie & Berntsen), 0.05 M TRIS-HCl buffer pH 8.7], 10 sec DEPC water and dehydration in ethanol as described above.
The spin-echo experiments were performed using 2 sec of water suppression prior to a 90 degrees excitation pulse.
The standard pulse-acquire experiments included a 60° flip angle over a spectral region of 20.0 ppm after 3.0 sec of water presaturation.
The air-dried cytospin slides underwent the following staining procedure: slides were immersed five times in 100% industrial methylated spirit (IMS Surgipathth Europe Ltd, Cambridgeshire), and then immersed in 70% IMS and washed for 30 sec in water.
Slides were rinsed for 30 sec using deionized water and then immersed twice in deionized water for 5 min. To detect Fas, tissue sections were initially treated with 1% SDS in TBS (100 mM Tris-HCl, pH 7.4, 138 mM NaCl and 27 mM KCl) for 5 min at room temperature, followed by three 5-min washes in TBS alone.
First, mice were allowed to swim for 60 sec in the water maze without a platform.
