Sentence examples for sec step from inspiring English sources

"We cooperated with the SEC step by step in this investigation," says eBay spokesman Kevin Pursglove.

Comparison of the analytical solution (3.5) and the numerical solution (3.6) of the fractional differential equation (3.1) for the parameters,, zero initial condition,  sec,, and is depicted in Figure 1. Figure 1 Comparison of analytical and numerical solutions of fractional-order viscoelastic models of cell (3. 3) for simulation time 5 sec, step, and in (3.6).

Samples from the affinity chromatography were used either directly or submitted to an additional SEC step.

Using Matlab (MathWorks, Natick, MA), a sliding window Fourier transform was applied to the LFP signal using a 2 sec window with a 1 sec step.

PCR was performed with a Bio-Rad iCycler and the following program: 35 cycles: step 1: 95° for 10 sec; step 2: 60° for 30 sec; step 3: 72° for 40 sec each cycle.

The following PCR temperature cycles were used (45 cycles were performed): Step 1 95°CC, 5 min; Step 2 95°CC, 10 sec; Step 3 55-60°CC (primer dependent), 10 sec; Step 4 72°CC, 10 sec; Step 5 4°CC, hold.

PCR reactions were either run on a 1605 Rapid Cycler (Idaho Technology, Idaho falls, ID) with a denaturation step of 1 min at 94°C followed by 35-40 cycles of 10 sec at 94°C, 10 sec at 5°C under the Tm of the oligonucleotide with the lowest Tm and 30 sec - 1 min at 72°C or on a BioRad C1000 thermal cycler using the same parameters except that 30 sec steps were used rather than 10 sec's.

The staining was conducted in an RNAse-free laminar flow hood and included the following 30-sec steps: 57% ethanol, distilled water, HistoGene stain (Arcturus, Mountain View, CA), distilled water, 75% ethanol, 95% ethanol, and 100% ethanol.

Reactions were performed in duplicate for each sample for 40 cycles (95°C/15 sec denaturing step; 60°C/1 min annealing/extension step).

The PCR protocol was 30 cycles of a 45 sec denaturation step at 94°C, a 1 min annealing step at 58°C and a 1-min extension step at 72°C.

A melt ramp consisting of 1°C increments from 60°C to 99°C (30 sec first step, 1 sec for subsequent steps) followed the 45 cycles.