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Additionally, patients randomly received MI-E in one of the sessions (one day intervention).MI-E treatment consisted in 4 series of 5 in-expiratory cycles at +/− 40 cmH2O pressure, 3 sec inspiratory time, 2 sec expiratory time, and 1 sec pause between cycle.
Slow tempo w/ 2 sec pause at the bottom.
Samples were disrupted by vortexing twice for 10 sec, intermitted by a 30 sec pause and clarified by a 20 min centrifugation at 12,000 g.
[4 sec pause] 2. S3: Oh, I have a comment.
Yes, but (3 sec pause, SK) what else can we do?
This was followed by three consecutive tone presentations (30 sec, 75 dB, 5 kHz), each separated by a 30 sec pause.
Similar(52)
Several two-second constant force pulses were applied to the cytoskeletal network through the injected magnetic bead with 30 sec pauses between the pulses.
Lysed cells were sonicated for 3×30 sec on ice with 30 sec pauses using a probe sonicator (Labsonic-M, 3-mm probe; cycle 0.5, 30% power; Sartorius AG, Goettingen, Germany, http://www.sartorius.com) to produce ∼400 500 bp chromatin fragments.
However, since our time between images in these experiments was 3-5 sec, pauses of less than 3 sec are likely included in the average speed.
Each worm pellet was resuspended in 10 volumes of Trizol. 1 ml of Trizol-worm mixture was transferred to a fresh screw-cap vial filled to 500 μ l with glass beads and homogenized in a Precellys homogenisator for 2 ×10 sec, 6000 rpm, 5 sec pausing.
Between two stimulations, at least a 30-sec pause was obtained to avoid superposition.
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