Exact(25)
"Just one sec," I said.
"Wait a sec," I mumbled, reaching for my bourbon.
They say, 'Wait a sec, I haven't seen a penny of this.
Ha ha ha, wait one sec — I have to text that to Emily".
Everybody walks by everybody's desk and like, "Hey, you have a sec?" I like to be able to participate in that.
But because of the Ivan Boeskys of the world, I soon learned that the Securities and Exchange Commission was not the SEC I grew up rooting for.
Similar(35)
Following amplification in E.coli, the expression cassette from pShuttle was excised using I-Ceu I/PI-Sec I homing enzymes and cloned into an Ad5F35 expression vector.
A - HPAEC elution pattern of xyloglucan from tamarind seed (2 mg mL-1) after incubation with partially purified Mt LPMO9A fraction SEC-I (Additional Figure 1 ; 12.5 mg g-1 substrate).
C - SDS-PAGE of marker (lane 1; Precision Plus Protein, Bio-Rad Laboratories), the crude enzyme extract (lane 2), pooled fraction AEC-I (lane 3) and partially purified fraction SEC-I (lane 4).
A - HPAEC elution pattern OS β-glucan before and after incubation with partially purified Mt LPMO9A fraction SEC-I (Additional Figure 1 ; 12.5 mg g-1 substrate), with addition of 1 mM ascorbic acid or without.
The MtLPMO9A preparation thus obtained (partially purified fraction SEC-I; Additional file 1) was further subjected (3rd step) to a Resource Q column (30 × 16 mm internal diameter, GE Healthcare), pre-equilibrated in 20 mM potassium phosphate buffer (pH 7.0).
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