Exact(3)
It currently offers 34 interconnected external and in-house tools, whose functionality covers sequence similarity searching, alignment construction, detection of sequence features, structure prediction, and sequence classification.
The database is available in ARB format, a sequence database management tool used widely in microbial ecology, allowing for point-and-click access to sequence record importation and exportation, searching, alignment editing, primer design and matching and phylogenetic tree construction (35).
The NCBI Protein Database accession numbers of complete or partial tenascin amino acid sequences identified by key-word search or BLASTP (NCBI Basic Local Alignment Search Tool [ 59]) (reviewed by [ 60]) and used to obtain data for searching, alignment and phylogenetic reconstructions are listed in Table 1.
Similar(57)
Small molecule bioactivities mapped to protein domains add important precision to approaches that use protein sequence searches alignments to assist applications in computational drug discovery and systems and chemical biology.
AS gave a contribution for polymorphisms searching and alignment of barley mature microRNA sequences.
An iterative procedure of HMMER search, alignment, manual inspection, and editing allowed us to construct full gene alignments, with annotated intron boundaries and BPs.
Partial sequences from clones and bacterial isolates were compared against the National Center for Biotechnology Information (NCBI) genomic database with the BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi) search alignment tool.
Tools such as Phylogenie (Frickey and Lupas, 2004) automatically identify orthologs of a starting sequence, via database searching, sequence alignment, alignment processing and tree reconstruction.
All authors (except NRI-V) contributed to the sequence data analysed, helped construct and edit alignments, and manually searched the alignments for microinversions.
Peptide sequence of target protein was searched for alignment using protein pilot software (Uniprot 2012).
MGL carried out the database searches, sequence alignment, phylogenetic trees performance and drafted the manuscript.
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