The PSSM contains amino acid evolutionary information from multiple sequence alignment searching against the SWISS-PROT database [19].
The ligands of the top ranked templates are used as template ligands for searching against compound library.
When searching against full-length templates, 7 to 9-mers provided the closest matches.
Searching against the whole human Uniprot dataset readily returned S. mansoni CRIT as the top hit.
The function of all coding sequences was further investigated by searching against InterPro database.
Functional annotation of CDSs was performed through searching against nr protein database using BLASTP [63].
Domain boundries were identified by searching against the PFAM database.
Protein domains were detected by searching against the Pfam database.
Bacterial similar sequences of each of these enzymes were also collected during searching against RefSeq_protein database.
KO of the putative transcripts (8837) were identified through BLAST searching against the KEGG database.
However, upon searching against individual genomes, the bacteriocin sequences are retrievable.
