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Steps that were parallelized included matching primers to sequence, and the Blast searches to align bovine with human sequence.
When using BLAST searches to align our 64-bp markers directly to the Danio rerio genome returned relatively few high-quality hits, we developed a strategy whereby we first aligned our GBS marker sequences to the Astyanax mexicanus genome and transcriptome data.
In order to annotate and evaluate the quality of our reads and of our assemblies, we performed BLASTX searches to align both contigs and singletons from each library with A. thaliana, Vitis vinifera and Populus trichocarpa proteomes and Uniprot (Tables 4).
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It's not pessimism, just cold hard facts: In over 25 years since the overthrow of communism, only a dozen or so cases filed by trans people searching to align their legal identity with their true self have been resolved in favor of the plaintiffs.
For most of these existing methods a hash-table must be built containing either the query (BLAST, MAQ and SHRIMP) or reference (SSAHA, BLAT and SOAP) sequences; this hash-table must then be searched to align reads.
BLAST searching was able to align 117 of the unknown proteins (Additional file 1).
Sequence similarity search algorithms used to align maps with Btau_2.0 have a considerable risk of errors as they may also detect gene duplications or similar motifs in different genes.
By utilizing the DrugBank database, druggability of non-homologous essential proteins of E. coli O157 H7 was measured by sequence similarity to the targets of small-molecule drugs and BLASTP search was performed to align the non-homologous essential proteins to the list of drug-targeted proteins from DrugBank.
The datasets varied by the similarity thresholds used in the profile searches [7], criteria used to align the sequences (profile, penalties), amount of gaps, and included from 217 to 2853 taxa (and from 64 to 217 aa in the alignment).
Protein- BLAST searches were then used to align sequences to each other, and redundant sequences were removed from the predicted transmembrane set.
In contrast, the segment-based method from our previous work took O(n) time to do an exhaustive search for the best segments to align the series.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com