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Besides the pre-computation expense to determine the reference shapes to use and to generate the 3-D fingerprint for all conformers to be searched, this method has an important drawback.
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Iteratively, we searched with this method as long as we were able to find new snoRNA homologs.
Diamonds reflect 85% of the light that hits them, so they will be very shiny when found! Surface searching: If you don't have a lot of time to spend at the Park or need an easy way to search, try this method.
If I had to search using this method, I would be giving up my greatest strength!
In contrast to conventional exhaustive grid search, this method can be treated as a deterministic analog of random search.
In addition to the intron retention search, this method was repeated for partial intron retention where an exon was extended to include the STEP.
To efficiently search this space, a method of feature selection termed Genetic-Race Search GRSS) [ 37] was recently used, combining a genetic algorithm [ 217] with RACE search [ 218].
On the other hand, the INFERNAL software applies to more general searches using alignments of both sequences and structures of seed RNAs; however successful searches using this method largely depend on the prerequisite that the candidate RNA is highly conserved at both sequence and structural levels with the seed RNAs used for the search.
Since this case involves only present people, all the problems discussed above will reappear in the search of this method.
For a complex problem with huge searching space, this method may encounter difficulty in finding the optimal solution and risk of divergence.
A new approach based on a single-reference-station mode has been published by Kim and Langley (2007) that nullifies the effect of the differential ionosphere in an ambiguity search process; this method provides a number of interesting results.
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