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We also searched target(s) for miR-221 in CAD late EPCs.
To identify miRNAs that are able to induce NF- κB signaling, we searched target prediction databases for putative target genes implicated in negative regulation of NF- κB using miRNAs that are overexpressed in GBM tissues.
To investigate whether these miRNAs directly interact with the two computational searched target genes, we co-transfected pGL3 vectors with p-miR-374a and p-miR-548b or controls in HEK293 cells.
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Further, we purposively searched targeted journals, including Functions of Language, Functional Linguistics, Languages in Contrast, Linguistics and the Human Sciences, Language Sciences, Language Typology, and Studies in Language.
Predictive algorithms have been developed to facilitate this process by computationally searching target sequences that bear the least similarities to other sequences to reduce the off-target effects.
TargetScan (http://www.targetscan.org/) and http://www.microrna.org/ databases were used to search target mRNAs [ 24, 25].
To search target genes, the conserved miRNA sequences were matched against the cotton transcriptome unigene database using psRNATarget (http://plantgrn.noble.org/psRNATarget/).org/psRNATarget/
SteerList uses a map where you can easily search "Target" and select the one you always go to.
Thus, the second design guideline is to distribute the search targets in an extended virtual reality environment so that children can search targets as they gradually move across the graphic display (background).
Additional citations were sought through the reference lists of relevant documents and hand searching targeted journals.
The standalone version of the plant target prediction tool permits one to choose transcripts databases for searching targets.
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