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The contigs were then searched for sequence similarity using BLAST [120] against the genbank non-redundant database [121].
Since most human genes have multiple promoters, individual promoters of such genes have their own entries in DBTSS that can be individually searched for sequence motifs.
We, therefore, initially searched for sequence motifs corresponding to optimal binding sites for PTB, with the rationale that negative feedback loops operating at the translational level might prevent overexpression of nPTB and ROD1.
We retrieved 10 kb-long contig sequences (5 kb either side of the hit), fragmented them into 2 kb segments (each overlapping by 1 kb) and searched for sequence homology of each fragment establishing a cut-off of E≤10−5.
Public databases on the Internet were searched for sequence homology.
We therefore searched for sequence gaps in regions of synteny with the cassettes from C. glabrata.
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The lamprey assembly was searched for sequences homologous to conserved noncoding sequences previously identified in comparisons between human and Fugu47 and human and Callorhinchus milii6 genomes.
To perform this type of phylogenetic analysis we searched for sequences similar to HET-D and HET-E in the fungal databases.
In this report, we searched for sequence-specific DNA-binding TFs using a prediction method which uses 51 Hidden Markov Models (HMMs) from the Pfam database.
Data were specifically searched for sequences related to carbohydrate metabolism.
This list was searched for sequences that were not found during the Entrez and BLAST searches.
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