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It searched for modifications that were found in two or more overlapping peptides, and took advantage of the reduced efficiency of tryptic cleavage at methylated residues [ 32], where overlapping peptides with missed cleavages were likely to be found.
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This sample allowed us to search for modifications of chromatin in senescent cells with highly compacted heterochromatin.
Genome-wide association studies of Gene-environment interaction (G
To determine if covalent modification occurred on the reactive E1 cysteines identified for DEDC in vivo, we searched for covalent modifications in E1-enriched HEK293 extracts using LC/MS/MS.
We searched for such modifications in HIF2α and HIF2α(1 485) clones, but could not detect any change in the typical cell shape nor in the number of neuronal extension (Fig. 2a).
We also searched for i) modification of translation starts that could arise because of an insertion of a methionine residue at GTG and TTG translation initiation codons, and ii) protein maturation consisting in the processing of the initiator Methionine, as described previously [ 23].
To evaluate the potential of glutathiotinylation, we also searched for +305 Da modifications, but no evidence for this modification was observed.
In addition, we searched for the same modifications on the heavy chain N-terminal peptide and found trace levels of the +174 Da adduct and no detection of the +156 Da adduct, thus we saw similar susceptibility as our stability sample (see Table 1).
Since it was shown that synovial fibroblasts (SF) are not only effector cells responding to inflammatory stimuli, but appear endogenously activated and potentially involved into spreading the disease [ 1], we searched for the epigenetic modifications leading to the activated phenotype of these cells.
Based on the identification of a thioether modification, all tandem mass spectra were re-searched for differential modifications on Ser and Thr residues by thiol-containing molecules such as GSH, Cys, Cys-Gly, and homocysteine.
Decades of searching for chemical modifications that might regulate dynein came up empty-handed.
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