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Data were analyzed using the MSD Chemstation software which was linked to the National Institute of Standards and Technology NISTT) mass spectral search program library ver. 2.0 d (2005; standard reference data program of the National Institute of Standards and Technology, USA) for peak identification of metabolites.
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The components were identified by their retention times and based on the commercially available spectral, (National Institute of Standards and Technology NISTT) mass spectral search program for NIST/EPA/NIH Mass Spectral Library V2.0) and mass fragmentation patterns using data of standards at Wiley 7.0 Library.
The resolved MS spectra were matched against reference mass spectra using the NIST mass spectral search program for the NIST/EPA/NIH mass spectral library (version 2.0) and our custom software for peak annotation written in JAVA.
Reference libraries (NIST/EPA/NIH Mass Spectral Library) from NIST MS search program version 2.0 were used to identify the MVOCs.
Mass spectra registered under the chromatograms were subjected to deconvolution, and then an identification of volatile compounds was carried out based on the reference libraries (NIST/EPA/NIH Mass Spectral Library) which are available in the NIST MS search program.
Proteins were identified by a local version of Mascot search program V2.1.04 and Mascot Daemon application V2.1.6 (Matrix Science Limited, http://www.matrixscience.com), using human sequence library in the International Protein Index (IPI) database (IPI_human_20080409, 72,340 sequences).
At the University of California, Berkeley, a professor and her students have created a search program called Flamenco that lets users browse a digitized collection in ways that are similar to a stroll among the shelves of a library.
The full library of standard programs contained over 400 programs in the Sinclair Program Library.
A total of 1,265 EST sequences, obtained cDNA library of M. avidus RNA, were analyzed by sequence comparison with previously reported sequences in the EMBL/GenBank databases using the BLAST X search program of the National Center for Biotechnology Information (NCBI).
de novo search programs.
Motif search programs were performed for the 70 motifs extracted at Step2-1 and the 4 MafB motifs at Step1-3 witheTFSEARCHandH and TFBIND algorithms, which are libraries of preexisting motifs for transcription factors.
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