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In the search for cell factors which support reverse transcription, in vitro biochemical systems provide a powerful means to analyze protein complexes and their activities, which are amenable to further advance by purification of components by chromatographic and other methods.
In a search for cell lines for high level expression of platelet-derived growth factor B (PDGF-B) from a transfected vector, the human prostate carcinoma cell line PC3 was found to be remarkably superior to many normal and tumour cell lines that were tested and the expression levels were on par with those in 293T.
In the search for cell cycle-specific phosphorylation of histone H1, human cancer cells or cells from species other than human have been used.
Whatever the technique studied, the search for cell groups and atypias gave results identical than those of LBC except for smears which showed a slightly higher percentage (p = 0.01).
In our search for cell models that are representative of a heterogeneous tumor environment, we developed two other cisplatin-resistant heterogeneous cell lines from an ovarian carcinoma model, A2780, as well as another NSCLC model, H2030 cells.
The zebrafish cardiac injury models provide a means to guide the search for cell types and pathways that can be driven to aid regenerative repair of cardiac function in mammals, and identify chemical modifiers to limit damage and/or stimulate regeneration across species.
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In addition, electrophysiological recordings could be made within the inner retina in a search for cells with the characteristic electrical responses of rod bipolar cells.
In a previously described search for cell-type specific genes of Volvox carteri [ 6], several gonidia-specific genes have been identified.
Additionally, filopodia are finger-like protrusions that – at a couple microns in length, a few hundred nanometers wide, and microns apart – extend even further from lamellipodium and search for cell-adhesive ligands in the environment [ 14].
In the first part of our approach, we designed a panel of simple binary "regressors" to search for cells with visual tuning profiles similar to the behavioral tuning.
We used coupled wide-field microscopy to search for cells in late mitosis or early G1 in which Sas-6 was either present or absent from nascent centrioles and then analyzed Plk4 localization with 3D-SIM.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com