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GL and DM did the search, assembly, predictions, cloning and sequencing of the cDNAs coding for the receptors and their ligands.
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CRIS is a bespoke database search and assembly tool which has supported a range of studies using this data set.
Additional homologues to yeast and E. cuniculi genes were identified using Blast (TBlastN, TBlastX, and BlastX; Altschul et al. 1997) to search the assembly.
Data for this study were obtained from these sources of clinical data in the SLaM BRC Case Register using Clinical Record Interactive Search (CRIS), a bespoke database search and assembly tool which has supported a range of studies using this data set.
The absence of CI in S. cerevisiae has been exploited in the search for assembly factors of this complex using a comparative genomics approach with CI-containing yeast species, such as Yarrowia lipolytica.
This method relies on the analysis of the frequencies of overlapping fixed-length sequences (k-mers) [ 16], thereby exploiting a computationally efficient and scalable analytical technique that underlies many search and assembly algorithms.
Discussions of k-mer length have treated it as a parameter to be optimized [ 28, 29] and as a sensitivity/specificity tuning parameter for search and assembly [ 28, 30].
This paper describes a system for performing content-based searches on assembly databases.
Further searching and assembly of soybean EST sequences revealed five other members of this gene family, namely GmPHD2 to GmPHD6 (DG973808, DQ973809, DQ973810, DQ973810, and DQ973811).
Working with existing data from ESTs in GenBank as a starting point, BLAST searches and assembly were used to create a cluster of 24 ESTs encoding actin (∼94% nucleotide identity).
ZG was primarily responsible for database searching and assembly of CDK genes.
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