Silver capsules were then sealed, loaded into a ThermoFinnigan TCEA auto-sampler, and introduced on-line to a ThermoFinnigan Delta Plus XP Mass Spectrometer through a Conflo III Interface.
Therefore, the load transmission relations between subsea connector components are first analyzed under different working conditions, and the relationship between the sealing contact load and the locking force is obtained.
Specifically, hyaluronic acid (HA) was applied as an end-capping agent to seal drug loads inside the mesoporous of MSNs through the acid labile hydrazine bonds.
Tubes were balanced, heat-sealed, and loaded into a 70Ti ultracentrifuge rotor (Beckman-Coulter).
After the microreactor was filled with immunocomplex solution and sealed (sample loading), the reactor was heated to 39 °C (heating).
The plate was sealed and loaded directly onto a Series 200 LC instrument (Perkin Elmer, Seer Green, UK) equipped with a refractive index detector.
The plate was sealed and loaded into a Gemini SpectraMax EM fluorescence plate reader (Molecular Devices, Sunnyvale, CA, USA), where it was incubated at 37 °C.
Data showed an increasing trend in 56 days for both crack sealing and load recovery.
With regard of sealing reliability of SPMC, loading force for installation, and minimal effect on pipeline strength, the sealing width, LAD, is 3 mm.
Including heat lysis step, reagent preparation, loading and sealing the card, and the typical ~20-min reaction time, the assay can be performed in less than 30 min.
