And came to London to sit day after day in this large box with the furniture to dust, and the muffled sound of private lives sealed away above, below and around her. Nazneen examined her finger.
Flasks were then incubated in a 95%air/5%% CO2 environment for 1 h before being tightly sealed to maintain the above environment during experimentation.
Twelve 85 µL replicates of a 10% w/v PEG 4000 solution were pipetted into the same 10 types of sitting drop plates and one hanging drop crystallisation plate used above, and sealed and incubated as described above.
Many in attendance struggled to contain tears as a local gravedigger placed and sealed the bodies in above-ground graves.
In fact, it fails so spectacularly that you get a sense that maybe even Sorkin, a reliable carrier of briefs for the hermetically sealed sects enumerated above, is starting to lose faith.
After staining, tumor sections were counterstained and sealed as mentioned above.
In one couple, the male's mouthparts were sealed as described above; in the other couple the male was untreated.
Samples were then shaken by hand for 2 min. Chicken was removed using forceps, and the bag was sealed 1 2 cm above the top of the broth.
Slides were incubated, rinsed, mounted and sealed as described above and digital images of optical sections through these samples were acquired with a Zeiss LSM 510 Meta Confocal System.
We used sterile forceps to transfer the entire piece of chicken to a stomacher bag containing 200 mL sterile Bolton broth (Oxoid, Hampshire UK) supplemented with laked horse blood (Quad Five, Ryegate, MT); samples were shaken by hand for 2 min, the chicken was removed using forceps, and the bag was sealed 1 2 cm above the top of the broth.
