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Membranes were washed once with 2 × SSC/0.2 % SDS and once with 0.1 × SSC/0.1 % SDS solutions at 62°C for 1 h, and were subsequently exposed to Kodak XAR-5 films at -80 C for 2 5 days.
Neutral salts at 0.1 0.3 M have negative preferential interaction parameter values in SDS solutions [31, 32], which means that these salts are preferentially excluded from SDS micelles.
The invariance of the half-wave potentials of ferrocene in pure SDS solutions whatever the surfactant and ferrocene concentrations suggests the use of this solute-solvent couple as a reference potential system.
However, to provide further insight as to how these FN nanofibers might compare to cell-generated FN fibrils, we repeated the release process in either 2% (w/v) deoxycholate (DOC) or 2% (w/v) sodium dodecylsulfate (SDS) solutions (Fig. 2a).
The storage capacity, induction time and induction temperature of CO2 hydrates were investigated on pure water, SDS solutions, THF solutions and mixtures of THF and SDS.
Several liquids with different physical properties, i.e. water, ethanol, three sodium carboxymethyl cellulose (CMC) solutions (0.0464%, 0.1262%, 0.2446% CMC) and two sodium dodecyl sulfate (SDS) solutions (0.0608%, 0.2610% SDS) are chosen as working fluid and nitrogen as working gas.
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Our findings from the CHP staining were further confirmed by the circular dichroism spectra of intact triple helical collagen molecules in CHAPS and SD solutions, and the TEM images of CHP-conjugated gold nanoparticles binding only to the SDS and Triton X-100 treated collagen fibrils.
Enzymic activity was stopped by adding 2 mL of SD solution.
Enzymic activity was stopped by adding 2 mL of SD solution [L15medium (Life Technologies) containing 0.52 mg mL−1 soybean trypsin inhibitor, 3.0 mg mL−1 BSA, and 0.04 mg mL−1 DNase (Sigma)] to prevent cell clumping.
One millilitre of 0.25% trypsin and 100 μL of 1.0% collagenase were added per mL of HBSS, and the mixture was incubated at 37°C for 20 min. One millilitre of SD solution was added per mL of HBSS, and the tissue fragments were broken down by gentle trituration (four times through a 21-gauge needle, and twice through a 23-gauge needle).
Each aortic tissue sample was treated with 20 ml of 1 % sodium dodecyl sulphate (SDS) (SD fine chem. limited, Mumbai) solution for 48 h at 37 °C with continuous shaking in an orbital shaker at the rate of 180 rpm.
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