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To detect histones in roots, proteins were extracted using 1× SDS sample buffer (without BPB).
The pellets and chromatin were resuspended in 2× SDS sample buffer.
The beads were eluted with SDS sample buffer and subjected to SDS-PAGE.
The cleavage reactions were stopped by adding SDS sample buffer and heating to 90 °C.
Reactions were stopped by addition of reducing SDS sample buffer and boiling for 5 min.
Agarose beads were washed three times with IP buffer and resuspended in SDS sample buffer.
TCL was prepared by adding 2× SDS sample buffer preheated at 65 °C directly to the wells.
Eluted protein was precipitated by TCA precipitation and dissolved into SDS sample buffer prior to resolution in a polyacrylamide gel.
The dried CNBr cleavage samples were resuspended in SDS sample buffer and neutralized by adding 0.5 M Tris-base.
For sample preparation, a 4 × SDS sample buffer was added, followed by boiling at 95 °C for 5 min.
The immunoprecipitated proteins were eluted from the beads by incubating with SDS sample buffer for 15 min at 95 °C.
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