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Those that remained were scrutinized visually and 29 027 SNPs with three clearly defined clusters were selected.
Ward's method resulted in a dendrogram, a graphical representation of the cluster building process, which was then scrutinized visually to find the optimum number of clusters, together with a mathematical criteria such as Caliński -Harabasz [ 27] or Duda Hart [ 28].
The remaining SNPs were scrutinized visually and those SNPs that did not show three clearly defined clusters representing the three possible genotypes (AA, AB and BB) were also excluded.
MEGA 4.0 was used for DNA sequence alignments and to visually scrutinize chromatogram trace files (Tamura et al. 2007).
Thus, the 1 % top-ranked molecules given by each program, which corresponded to 143 different molecules per program, were visually scrutinized.
First we tested different thresholds by 1% lag between 95 and 99% and visually scrutinized the patterns of ZVEEC for each threshold in different conditions (day-night, offshore-inshore).
We further visually scrutinized the entire 1.15 Mb region of autozygosity.
The output files were readily uploaded in the Integrative Genomics Viewer (IGV) (Robinson et al., 2011) and visually scrutinized for private variation.
Functional datasets were visually scrutinized for evidence of sustained head motion, severe image distortion, and field-of-view misalignments, and corrupted data were discarded accordingly.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com