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Perl scripts for extracting proper data from source result files and generating plots were developed in-house.
The Python script for generating the simulation data and Perl scripts for extracting and pre-processing of these SNP data are available in Additional File 1.
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We thank several colleagues at the Marine Biological Lab: Adam Lazarus collected ant specimens and obtained Blochmannia trpE sequences, James Style prepared genomic DNA, Rich Fox installed and compiled many of the bioinformatics tools used here, Sheri Simmons provided the script for extracting intergenic sequences, and Gretta Serres offered helpful discussions of functional annotations.
Additional file 3: Perl script used for extracting intraspecific SNPs.
Additional file 4: Perl script used for extracting interspecific SNPs.
This was due to a modification to the script used for extracting sequence alignment profiles from the dataset [ 2].
A script was developed for extracting the volcanic ash attenuation relationship (changing ash load with distance) for each hazard footprint generated by the dispersal model in the synthetic catalogue (Fig. 6).
Then, Perl script was written to scan the files for extracting the information of MITEs close to or in predicted genes.
We wrote PERL scripts to extract sequences for each region between a pair of replication origins and further prune them to only consist of non-coding nucleotides and the synonymous third codon position nucleotide, corresponding to an 'A' or a 'G'.
In addition, we have also developed a software tool (using open source scripts) for use in extracting and analysing the data from the GMAP.
Blast alignments were then parsed with custom python scripts to extract and count the best miRNA alignment for each read with a minimum read alignment of 18 nucleotides.
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