The R script for detecting CTLP cases can be provided upon request.
This unigene dataset was used for detecting SSR loci by a Perl script known as MicroSAtellite (MISA, http://pgrc.ipk-gatersleben.de/misa) and primers were designed using Primer 3.0 [ 29].
The mapping results are input into script, Maq2rlt, Soap2rlt, Ssaha2rlt, or Smalt2rlt in the SEG-Map package for detecting SNPs of the mapping individuals.
The alarming tool that we currently use (Script L3; Additional file 7) is very basic and uses a simple temperature threshold, but more sensitive techniques for detecting subtle changes are possible.
Strategies for detecting liars.
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