Scripting consists of constraints that structure conversation or discourse among collaborators with the aim of guiding the exchange of knowledge and information (Kirschner et al. 2008).
The model hSR-AI structure and either the human apo A-I structure (PDB entry 2a01) or the human apo E structure (PDB entry 1ea8) were docked together using the suite of Haddock scripts (31), with constraints on docking residues of SR-A as follows: Arg325, Lys332, Lys335, and Lys338.
The complete UniProtKB/Swiss-Prot protein sequence database and the whole set of peptides predicted from the fully sequenced genomes of C. gigas (release 9) (Zhang et al. 2012) and P. fucata (v 1.0) (Takeuchi et al. 2012) were screened for the presence of the C-C-CC-C-C pattern with a custom Perl script, without any constraint about the spacing between cysteine residues.
A major removal from the script, due to time constraints, was a long sequence titled "Night of the Demons": the main character, a shop assistant named Stephen Baxter who discovers his divine lineage, takes over a hotel with his disciples and eventually encounters several of the hotel's employees that have been possessed by the Devil.
The patient was described as 'mute statue' imposing and significant, and yet also visibly powerless in her muteness, and the doctor as the orchestral conductor, working to bring together different concepts within an institutional script that, nevertheless, imposes constraints on what is possible for him to do.
Driven by off-the-wall characters pursuing eccentric passions, this "harebrained idea of a film" (according to Mr. Godshall, in his manifesto) erupted from a desire to cast off all constraints — including a script — and go with the flow.
FastReader allows exporting of the schema definitions such as tables, constraints, sequences, triggers and generating scripts.
The codon substitution analysis was carried out using CodAlignView (Jungreis I, Lin M ,Kellis M. CodAlignView: a tool for visualizing protein-coding constraint) and processed with a python script to remove sequences of codons.
She accepts constraints — hitting her marks, sticking to the script — so listeners might break free of theirs.
