These PPRHs were tested in PC3 prostate cancer cells in an in vitro screening of cell viability and apoptosis.
Large-scale single-cell arrays are urgently required for current high-throughput screening of cell function and heterogeneity.
Here, we describe an approach for the combinatorial screening of cell adhesion behavior to gain understanding of how ECM protein feature size dictates osteogenic differentiation of mesenchymal stem cells.
Using this assay, we measured apparent KM and kcat values for DAAT and identified mutants displaying altered substrate specificity via the screening of cell lysates in 96-well plates.
Thus, routine screening of cell lines for mycoplasma is important for the maintenance of reliable experimental data and contaminated cell lines can be restored to their baseline function with antibiotic clearance of mycoplasma.
Our further screening of cell cycle factors identified the cyclin-dependent kinase inhibitor p57 as the transcriptional target, and detected a responsive element of C/EBPβ in the promoter.
To define a cut-off expression level to delineate high and low expressors of the IFN/STAT1 pathway we developed a method for rapid and efficient screening of cell lines and tumor clones.
The number of inside cells (corresponding to the number of asymmetric divisions during the previous mitosis) was measured in a population of 78 fixed 16-cell stage embryos stained for chromatin and actin to allow an easy screening of cell periphery.
Therefore, it is ideally suited for screening of cell cycle-modifying substances in CMs.
(A ) Screening of cell cycle inhibitors for enhancement of TE and HDR frequencies in human primary neonatal fibroblast cells.
