Sentence examples for screens affinity from inspiring English sources

High-throughput studies using yeast 2-hybrid screens, affinity capture coupled with mass spectrometry and other physical methods have pointed to extensive protein:protein interactions among the eight RAD52 group proteins that are required for resistance to EcoRI (Table  5).

Physical interactions based on 2-hybrid screens, affinity capture with mass spectrometry and related methods were evaluated, but associations inferred based only on co-localization experiments were not used.

Using the HRS-based FP setup, we were able to screen affinities of off-line-generated metabolites of zearalenone for ERα.

The aim of this study was to use a systematic approach to generate and screen affinity-purified, mono-specific antibodies targeting progression-related biomarkers, with a view towards developing a clinically applicable IHC-based prognostic biomarker panel for breast cancer.

These findings further exemplify the difference between interactions detected by Y2H screens and affinity purification procedures.

To identify interaction partners of a protein of interest, several large-scale methods such as yeast two-hybrid screens, tandem affinity purification followed by mass spectrometric analyses, and protein chips are commonly used.

Higher confidence data can be obtained by integrating results from both approaches, as protein interactions that are detected in both two-hybrid screens and affinity purifications are estimated to be five to ten times more likely to occur in vivo than those detected using only one method (von Mering et al., 2002).

Currently, the available interactomes are primarily formed by protein-protein interaction (PPIs) networks in which the interactions have been experimentally obtained either from high throughput experiments (such as large-scale two hybrid screens and affinity purifications/mass spectrometry [ 2- 12]) or by different types of low-scale experiments.

In recent years multiple efforts, mainly employing yeast two-hybrid screens or affinity purification followed by mass spectrometry (AP-MS) have been undertaken to construct high confidence interaction networks of human proteins (Venkatesan et al. 2009; Glatter et al. 2009; Kristensen et al. 2012; Hubner et al. 2010; Mellacheruvu et al. 2013; Sowa et al. 2009).

We present herein a novel bioseparation/chemical analysis strategy for protein ligand screening and affinity ranking in compound mixtures, designed to increase screening rates and improve sensitivity and ruggedness in performance.

All prepared compounds were screened for affinity for the Arabidopsis thaliana cytokinin receptor (CRE1/AHK4).