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19 Monte Carlo simulations of chlamydia positivity before and after screening in hypothetical clusters (assuming that positivity ranged from 0 to 10% with an average of 4%, 5%, or 6% before screening) were run to determine the minimum detectable decline with given population numbers for the three regions and for each block.
Assays locally used by the blood bank for HBV and syphilis screening were run in parallel to quality-assured external assays supplied during the study, and all discordant samples were submitted to confirmation testing in reference laboratories in Mozambique and Belgium.
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When there was inconsistency between two runs, a third screening was run to ensure the correct assignment of infection status.
Genotyping assays for the initial screen were run on the Illumina BeadArray platform (www.illumina.com).
These screens were run for 3 weeks (i.e. approximately ten asexual generations).
All screens were run on a LSRII + HTS BD Bioscsiencess) flow cytometer.
All screens were run with a positive control of DNA from known infected individuals of other insects, so this failure is unlikely to be due to technical PCR error, although we cannot completely rule out the presence of a divergent strain of one of these bacterial taxa.
The scoring window that a fan sees on his computer screen is run by a small piece of computer code known as an applet.
The virtual screen was run once, then the median throughput (in molecule/s) of the five subsequent runs was computed.
First, basic screens are run on blood and urine to detect categories such as opiates, amphetamines, marijuana, alcohol and barbiturates.
The primary screen was run in duplicate on two separate days.
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