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To allow the investigation of different factors, a screening was conducted using the BioLector® microfermentation system (m2p-labs, Germany).
Virtual screening was conducted using the three X-ray crystals 3VRI, 3VRJ, and 3UPR in the presence and absence of peptide.
Participant screening was conducted using a telephone questionnaire.
Cognitive screening was conducted using the validated IDEA cognitive screen (11).
HIV screening was conducted using Determine® HIV-1/2 (Abbott Laboratories, United Kingdom).
On-site couple verification screening was conducted using a somewhat different protocol.
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If the patients are interested in participating in the trial, a telephone screening is conducted using the PSQI to clarify whether they fulfill the criteria for poor sleep (PSQI global score >5).
During the period from 2000 to 2005 (64% of the included patients) genetic mutation screening were conducted using a setup consisting of denaturing high-performance liquid chromatography (DHPLC), protein truncation test (PTT) and Sanger sequencing analysis in addition to Multiplex Ligation-dependent Probe Amplification (MLPA) for detection of larger copy number abnormalities.
PCR-screening was conducted using DNA pools prepared with three-dimensional sampling method from approximately 40,000 plants bearing Tos17 insertion (Agrawal et al. 2005).
A screen was conducted using dsRNAs encoding cullin-1 (Cpcul1), maleless (Cpmle), musashi (Cpmsi), a homeobox gene (CpHbx), and pumilio (Cppum).
This new mutant screen was conducted using a T-DNA mutagenized Arabidopsis thaliana var.
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