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This screening verified tandem SNP haplotype frequencies in three different pools, each with 96 individuals, by high-fidelity PCR and CTCE as previously described; this analysis also demonstrated that the assay had no allelic bias during amplification [17], [18].
This screening verified absence of recombination in sequences before running the selection analyses.
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Duncan Greive, the editor of the New Zealand entertainment website the Spinoff, saw the episode on Friday in an exclusive screening and verified the incident occurred.
Recombinant plasmids were transformed into Escherichia coli, selected by blue/white screening, and verified by PCR.
ELISA and ECL samples found positive in screening were verified by a second confirmatory assay and titre determination.
Apoptin-interacting partners were obtained by yeast two-hybrid screening and verified by immunoprecipitation assays in mammalian cells, as previously described by Danen-Van Oorschot et al. Positive clones from the yeast two-hybrid screen were digested with XhoI to generate cDNA fragments, and subcloned into pMT2SM-myc to provide the fragments with an in-frame N-terminal myc-tag.
At the time the population for this study was selected, there were 145 children with screening-detected/biopsy verified CD and 62 who reported having CD diagnosed prior to the screening and gave a blood sample.
TMPZ, Dihydroergotamine, harmine and 1, 2, 3, 4-tetrahydroharmine were screened and verified (as shown in Additional file 1: Figure S2).
"Hits" identified from the screens were verified by FACS (Fig. 1C, E).
In the present study, we screened and verified OAZ mediated global hypomethylation of genome DNA and histone H3 and H4 tails.
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