Sentence examples for screening the crystallization from inspiring English sources

Two salts of the anti-human immunodeficiency virus (HIV) drug lamivudine namely, lamivudine hydrochloride and lamivudine hydrochloride monohydrate, were prepared in the course of screening the crystallization conditions of lamivudine duplex, an uncommon DNA-mimic, double-stranded helical structure made up of partially protonated drug pairs.

It is essential that the protein samples for screening the crystallization conditions are identical, which is especially critical at the beginning of the project.

In our previous publication [33], we presented a variable temperature strategy to screen the crystallization conditions, which showed that the crystallization success rate of thaumatin is not sensitive to the temperature.

In this study we found that nanospheres in combination with a commercial crystallization screen increase the crystallization success when screening crystallization conditions and that nanospheres can also be used as an additive in the fine-tuning of crystallization conditions, especially for problematic crystals.

The use of nanospheres in conjunction with the screening of the crystallization conditions was tested with HEWL, XYNII and XI by using the 50 nm nanosphere solution and the Crystal Screen HT solutions, purchased from Hampton Research.

Automated crystallizations with 96-well plates [ 35, 42] and robotic screening of the crystallization results can speed up this step [ 43– 43].

The production of concentrated solutions of the protein containing little or no detergent provides a means for screening crystallization conditions and the selection of detergents that facilitate crystallization.

Stocks of PCNA and dsDNA solutions were mixed to final concentrations of 0.4 mM and 1.1 mM, respectively (1 8 trimer:duplex molar ratio), and incubated at room temperature for 30 min before screening crystallization conditions by the hanging drop vapour diffusion method.

All in all, the use of nanospheres in protein crystallization proved to be beneficial, both when screening new crystallization conditions to promote nucleation and when used as an additive to produce better quality crystals, faster.

This chapter focuses on basic protein chemistry, variables affecting solubility, mechanisms of growth rate, and the typical means of screening crystallization conditions.

Protein crystallization remains difficult to rationalize and screening for optimal crystallization conditions is a tedious and time consuming procedure.