Sentence examples for screening substrates from inspiring English sources

Promising mutants were probed using both diastereoisomeric screening substrates (4 S)- and (4 R)- 4 in order that genuinely selective enzymes were identified.

About 1% of library members showed significant differences in activity between the two diastereoisomeric screening substrates, (4 S)- and (4 R)- 4. Two variants were selected as displaying greater stereoselectivity than the E192N 'parental' variant.

By coupling the technique of SECM with a light source, we have examined the surface charge of non-earthed pseudo-DSCs under variable light conditions and screened substrates by varying the working electrode potential.

The screened substrates were then stored in airtight plastic bags at room temperature until use.

Finally, using high-content screening methods incorporating GFP-fused cargoes, it might be possible to screen substrates of CDK1 115 for those that affect the protein transport process.

Primary cultured monolayers of rat type II-like pneumocytes appear to be a useful tool for screening MRP1 substrates designed for pulmonary delivery/targeting.

The DNA polymerase does not merely facilitate the insertion of dNMP, but it also performs rapid screening of substrates to ensure a high degree of fidelity.

Comprehensive screening of substrates for kinases is necessary to increase understanding of the signaling networks in which protein kinases participate.

As an extension of this method, genome-wide screening of substrates for 87 yeast protein kinases has been performed using protein microarrays containing 4,400 yeast proteins [1].

To examine whether this method is useful for screening novel substrates, not only in vitro but also in vivo, we focused on DCX, because the role of DCX and its phosphorylation by PKA and Cdk5 has been elucidated [9], [10].

Combining this simple method with a conventional method, such as 2D-DIGE, would be more informative and powerful for screening novel substrates of specific kinases and would increase understanding of the phosphorylation network.