Sentence examples for screening strain from inspiring English sources

The requirements for an effective high throughput screening strain include high frequency of transformation, reliable gene expression, reproducible growth in microtiter plate (MTP) format, sufficient and reproducible protein production, and low secretion of background proteins.

The S. cerevisiae screening strain TMB3044 that has been optimised for efficient pentose utilization, but which lacks the initial conversion step from xylose to xylulose [ 26], was transformed with the three constructed plasmids in addition to the empty plasmid to generate strains TMB3363 empty plasmid), TMB3359 XiPiromyces), TMB3364 (xym1) and TMB3365 (xym2).

Bacterial fermentation products have been examined for possible use as renewable energy and materials in the biorefinery industry [78] and therefore, RT-MT might be extended to strain screening, strain improvement, and evaluation for metabolic engineering [36], [79] [81].

After initial screening, strain TH13/00 (S. flexneri 2a) was chosen as a positive control for PCR assays.

UAS-Dcr2 was included in the screening strain for the purpose of enhancing RNAi-mediated knock-down by long dsRNA hairpins (Dietzl et al., 2007).

Furthermore, the haploid yeast strains with HIS3 and LEU2 plasmids were mated to make a diploid screening strain control without YACs.

Y.L., H.X., and H.Z. worked on SCRaMbLE experiments screening strains in environmental and drug conditions.

Our approach entails selecting knockdown target genes resulting from in silico flux response analysis and those related to product biosynthesis then screening strains transformed with a library of synthetic sRNA-promoter combinations for enhanced production.

The additional changes in chromosome copy number were detected by screening strains that were passaged in culture or through mice.

Therefore, the capability to utilize xylose to accumulate lipids is a critical criterion for screening strains with industrial potential in biodiesel area.

Previously, by screening strains from the N. crassa deletion collection (Colot et al. 2006) for sensitivity to staurosporine, we observed that a strain lacking NCU09974 was highly susceptible to the drug (Fernandes et al. 2011).