Many flavors of ABPs have been created, including photoactivatable, radioactive, and biotinylated (105, 106), which greatly facilitates both discovering novel enzymes and inhibitor screening (reviewed in refs (54) and (107)).
In addition to the powerful tools available for genetic manipulation, the small size (<5 cm) and prolific reproduction (200 300 progeny/pair/week) make zebrafish highly cost effective and uniquely suited for high-throughput in vivo screening (reviewed in Meeker and Trede, 2008).
The review process was done by a multidisciplinary team: one author conducted the information retrieval (incl. pre-screening) while screening, reviewing and summarizing the results were done by all authors - inter alia an ethicist.
During the Family and Preventive Medicine clerkship, students review with faculty the criteria for AAA screening, review the ultrasound AAA protocol, and practice scanning the aorta.
Similarly, the United States Environmental Protection Agency US EPAA) has published guidelines for screening, reviewing, and using published open literature toxicity data in ecological risk assessments [6]; however, these lack detailed guidance regarding relevance evaluation.
These reports are screened, reviewed and validated by in-country infectious disease experts before posting to the website.
Helicases in general have received recent drug-discovery attention, and small molecule inhibitors of viral helicases have been the focus of several high throughput screens (reviewed in [ 111]).
When comparing results from several RNAi screens [ 23, 32– 35], Stertz and Shaw identified 34 genes with potential importance for viral replication that were found in at least two screens (reviewed in [ 20]).
The identification of KAP1, SETDB1, and DNMT1 as ZNF304 corepressors indicates that our shRNA screen, like other large-scale shRNA screens (reviewed in Mullenders and Bernards, 2009), was not saturating.
Despite much study, the genetic pathways that direct early gonadal development and establish sexual dimorphism in the gonad remain largely unknown, with just a handful of regulatory genes identified so far from genetic screens (reviewed by Emmons 2014).
Mutations in KRAS, NRAS and PIK3CA, all of which have previously been identified in biliary tract carcinomas (for summary of mutations in BTC see Table 4), were identified in this mutational screen (Reviewed in [ 23]) [ 3, 6, 24, 25].
