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Thirteen screening reaction conditions (total of 160 syntheses) produced 11 different known molecular sieves.
Supported Pt and Ru mono- and bimetallic alloy catalysts, with promising potential applications in biomass processing, were investigated for their tolerance to sulfur poisoning using the batch hydrogenation of cyclohexene to cyclohexane in the presence of thiophene as a screening reaction.
In addition, each screening reaction had a positive control, the male-killing Spiroplasma infecting D. melanogaster [11].
SDH staining is regarded as the standard screening reaction for mitochondria [ 14].
Using this injection method, screening reaction samples containing substrate, product, and rhodamine were separated with good efficiency.
After intensively screening reaction conditions employing the [RuCl2 p-cymene)]2 catalyst without any significant improvements (not shown), it turned out that [RuCl2 p-cymeneve better conversion (Table 1, entry 3).
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Ten parallel photocatalyst screening reactions were achieved within 15 min in the multi-channel chip.
Screening reactions were carried out in Microfluor 2 black U-bottom 96-well plates (Fisher Scientific).
Screening reactions were amplified with an initial denaturation step at 94°C for 3 minutes followed by 30 cycles of 94°C for 30 seconds, 50°C for 30 seconds and 72°C for 45 seconds, ending with 72°C extension step for 7 minutes.
A potential approach to HTS by droplet MS is to complete entire screening reactions at the droplet level yielding both label-free detection and miniaturization.
For studies aiming to anchor genetic markers into physical maps of large genomes, the minimum number of screening reactions is decisive in setting labour and consumables costs.
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