Screening of the samples consisted of PCR with a pair of primers as described by Dalton et al. (4 ).
Screening of the samples was performed by amplifying a 251 bp fragment of the polymerase gene using the following primer set: Cor-FW (5'-ACWCARHTVAAYYTNAARTAYGC-3') and Cor-RV (5'-TCRCAYTTDGGRTARTCCCA-3').
A contract laboratory, Telelab (now Unilabs Telelab, Skien, Norway), performed the initial screening of the samples using real-time PCR to detect B. divergens DNA published by Radzijevskaja et al. [ 17].
To chart genomic alterations in our sample set, we first performed a high-resolution genome-wide screen of the samples using the SNP arrays.
Screening of the Merck sample collection identified compound 1 as a weakly potent GPR119 agonist (hEC50 = 3600 nM).
We produced between 10 and 15 screen captures of the sample in a grid, with a magnification of (times)500.
As a result, a sample of 574 households was selected in the study after these protest bids are screened out of the sample.
Subjects who did not pass the literacy screen (18% of the sample) were assigned a score of zero.
Equal fractions of individual samples are mixed from each population separately, and afterwards qualitative and semi-quantitative screening of the composed samples can be conducted.
None of these patients were included in the initial screening of the PBMC samples.
