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PCR conditions: One cycle of 95°C for 1 min, 50°C for 1 min, 72°C for 1 min. For methylation screening of the candidate tumor suppressor gene (TSG) BTG2 we used Ms-MCA as described [19].
Human peptide transporter: SLC15A1 Human Glypican5: GPC5 MM carried out mutation screening of the candidate genes and drafted the manuscript.
Screening of the candidate stressors can be accomplished by applying statistical tools (Norton et al. 2002) and strength of evidence approaches such as outlined in the SI process.
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But of course, as I said before, the screening of the candidates by the Council of Guardians is now under way and is crucial.
To exclude nonspecific DNA-binding inhibitors, we next performed a counter screen of the candidate compounds that were confirmed in the dose response test.
With screening methods of the candidate cDNA to express lentiviral vectors in IRF3‐deficient BMDC, it is possible to identify NK activation molecules.
Through RNAi screening of the 120 candidate progeny transcripts, we identified mex3-1 as a critical factor in cell fate decision-making.
Through RNA interference (RNAi) screening of the 120 candidate transcripts, we identified a homolog to the RNA-binding protein MEX3 (Smed-mex3-1) as a critical regulator of postmitotic stem cell progeny.
This was combined with simultaneous M cell expression of RNAi constructs to screen for the candidate signal molecule.
For screening of the study candidates, the patients' medical records were checked regarding gender, body size, and cardiovascular information, so that small and non-small hearts were evenly distributed among the studied patients.
Additionally, efficient processes to obtain VHH binders currently rely on camelidae immunization, followed by phage display selections from 'immune' libraries and screening of the best candidates.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com