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In a systematic screening of the activities of the 11 human zinc-dependent lysine deacetylases (i.e., HDAC1 HDAC11) against a series of C-terminal lysine acylated peptides, Olsen et al. found that HDAC3 in complex with nuclear receptor corepressor 1 (hadC3–NCoR1) hadetectablele decrotonylase activity towards a model peptide substrate in a fluorometric assay (Madsen and Olsen, 2012).
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We therefore recommend caution in comparing the screening yields of the activities reported here.
In our screen of the activity profiles of the TPR proteins Cyp40, like FKBP52, had no or very little effect on steroid receptors (Figs. 3 and 4).
The present study was thus aimed to the—(1) development of high frequency micropropagation method, (2) screening of the antimicrobial activities of different solvent-extracts of B. monnieri against various pathogenic strains of MDR bacteria and standard laboratory strains (MTCC) of bacteria and fungi.
In this study ABTS decolorization assay, ferric reducing antioxidant power, and ability to lipid peroxidation inhibition were used for screening of the antioxidant activities of analyzed samples.
The primary screening of the antifungal activity among these strains has shown antifungal activity of lactobacilli strains MDC 9661, INA-5.1, INA-21.1, RIN-2003, MDC 9632 and MDC 9633 (Table 1).
This methodology is nontoxic, uses a thermally-stable reagent and is suitable for random screening of the antimycobacterial activity.
For a quick screening of the antimicrobial activity, the cross steak method was used as described by Hemashenpagam with some modifications (Hemashenpagam 2011).
Screening of the anticancer activity of the target compounds revealed that the selected NO-donating compounds exhibited from mild to strong cytotoxic activity.
Preliminary screening of the antimicrobial activity in vitro of the essential oils from D. muricatus species against nine pathogenic microorganisms were studied using the filter paper disc agar-diffusion technique.
The in vitro qualitative screening of the antimicrobial activity was carried out by an adapted agar diffusion technique using a bacterial suspension of 0.5 McFarland density obtained from 24-h cultures.
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