Sentence examples for screening of gene from inspiring English sources
Comparative screening of gene expression libraries employing the potent industrial host Pichia pastoris for improving recombinant eukaryotic enzymes by protein engineering was an unsolved task.
With a comprehensive selection of genes of interest, we can focus on key signalling pathways and molecular mechanisms at relatively low cost compared to commercial platforms which are usually targeted at global screening of gene expression.
In previous studies, our laboratory successfully established a membrane array operation platform with a diagnostic biochip for the screening of gene overexpression by circulating tumor cells in cancer patients.
The method enables quantitative screening of gene libraries to identify clones with improved activity and it also allows cells to be selected based not only on the overall activity but also on the specific activity of the enzyme.
This biosensor has been successfully applied in analyzing and engineering metabolic flux in the shikimic acid pathway, through genome-wide screening of gene targets critical for the pathway flux, and by improving the specific activity of pathway key enzyme, AroG.
These results indicate that siRNA is a highly specific tool for targeted gene knockdown and for establishing siRNA-mediated gene silencing, which could be a reliable approach for large-scale screening of gene function and drug target validation.
Our gene panel allowed us to perform a very rapid and cost-effective screening of genes involved in Charcot-Marie-Tooth disease/hereditary motor neuropathy.
DNA microarray of non-viral reverse transfection in cell engineering allows drastic downsizing of large-scale functional screening of genes and siRNAs.
No band was seen in any of the negative control experiments lacking DNA. Figure 4 PCR patterns of B. subtilis and B. cereus for screening of genes coding for toxins.
Gene expression microarrays have been used particularly for screening of genes involved in specific biological processes of interest, such as diseases or responses to environmental stimuli.
Therefore, the versatility of CARS microscopy is critical not only for forward genotype-phenotype screening of genes that control fat accumulation, but also for the studies of potential interactions of lipid metabolism genes.
