This work also establishes a robust assay for the screening of compounds aiming to reduce SOD1 aggregation.
Cho, S. H. et al. Low-oxygen-recovery assay for high-throughput screening of compounds against nonreplicating Mycobacterium tuberculosis.
These findings open new windows for the design and screening of compounds targeting eIF4E.
Microarray technology offers the potential for mass screening of compounds on a scale, which was once unimaginable.
Throw in improvements in the rapid screening of compounds for biological activity and toxicity, as well as improved computational design tools, and an automated system could soon take over much of the drug discovery process, says MacKenzie.
However, wider application of this methodological approach to more differentiated cells such as neurons would also be useful for the screening of compounds that modulate synaptic transmission.
Random screening of compounds in endothelin receptor (ETA and ETB) binding assays led to the discovery of a new class of pyrazol-5-ol ligands.
Preliminary antidepressant screening of compounds (4a p) revealed that compounds 4b, 4c, 4d, 4e, 4i and 4o significantly (P < 0.01) reduces the duration of immobility time.
The use of multiple extraction temperatures for the HS-SPME procedure proved to be an excellent alternative for the screening of compounds present in honey with a wide range of volatilities.
Through directed screening of compounds prepared as metalloprotease inhibitors a compound, CGS 30084, that had potent endothelin converting enzyme-1 (ECE-1) in vitro inhibitory activity (IC50=77 nM) was identified.
Considerable progress in docking algorithms has enabled in silico screening as an attractive alternative to traditional screening for drug leads and optimization, because in vitro high-throughput screening of compounds is costly and relatively inefficient.
