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These PPRHs were tested in PC3 prostate cancer cells in an in vitro screening of cell viability and apoptosis.
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The induction of apoptosis and resultant loss of cell viability can be assessed by a duplexed fluorescence microplate-based assay, suitable for high-throughput screening (HTS) applications.
Indeed, treatment with BI-2536 and BMS-536924 showed significant loss of cell viability (cell viabilities of 37.77% and 47.15%, respectively; Figure 5C) in cell line screens.
The data are presented as percentage of cell viability relative to unstimulated cells (0 μM).
Sensitive detection of cell necrosis is crucial for the determination of cell viability.
Cellular ATP content was determined as a marker of cell viability using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Sweden).
Cell viability data were expressed as percentage of cell viability calculated.
Determination of cell viability.
Results expressed in percent of cell viability.
Restoration of cell viability was analyzed.
Similarly, reporter gene-based and lethality-based screens of Caspases in yeast could be applied in tandem for compound library screening, using various colorimetric and fluorigenic indicators of yeast cell viability for the latter in microtiter plate format.
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