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These PPRHs were tested in PC3 prostate cancer cells in an in vitro screening of cell viability and apoptosis.
Large-scale single-cell arrays are urgently required for current high-throughput screening of cell function and heterogeneity.
Using this assay, we measured apparent KM and kcat values for DAAT and identified mutants displaying altered substrate specificity via the screening of cell lysates in 96-well plates.
Here, we describe an approach for the combinatorial screening of cell adhesion behavior to gain understanding of how ECM protein feature size dictates osteogenic differentiation of mesenchymal stem cells.
Our further screening of cell cycle factors identified the cyclin-dependent kinase inhibitor p57 as the transcriptional target, and detected a responsive element of C/EBPβ in the promoter.
Thus, routine screening of cell lines for mycoplasma is important for the maintenance of reliable experimental data and contaminated cell lines can be restored to their baseline function with antibiotic clearance of mycoplasma.
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The living radical component of this CLiPP device fabrication platform enables facile incorporation of 3D culture sites into microfluidic cell culture devices, which can be utilized for high throughput screening of cell-material interactions.
High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described.
This spring Tsunoda hopes to have the first results from a systematic screening of cells, including a second batch of cloned calves produced using cells from 20 different tissues, including the liver, kidney, and heart.
This clearly demonstrates the benefit of smaller wells for efficient screening of cell-cell interactions unless cells are forced to aggregate, e.g. by using ultrasound [37].
Screening of cells expressing Sn and/or CD163 was performed with a fluorescence microscope (Leica Microsystems GmbH, Heidelberg, Germany).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com