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The screening medium without cell inoculation served as the control.
The screening medium (unless otherwise specified) was used in all the optimization experiments mentioned above.
These concentrations are within the measured values, and were appropriate as the screening medium.
The additive agents used were glycerol (2 % v/v) and calcium chloride (2 g/L basal screening medium).
In this screening, the diameters of the clearing zones surrounding the wells on the plate screening medium ranged from 11 ± 0.4 to 77.5 ± 7.4 mm.
The work function difference between the two surfaces of a slab is compensated by an effective screening medium method proposed by Otani and Sugino [12].
The residual NH4 +-N (from (NH4 2SO4 in the screening medium) was detected according to the method described previously (Yang et al. 2006).
(iv) To determine the most suitable pH for NH4 +-N degradation, HCl or NaOH was adopted to adjust the initial pH of the screening medium to 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, and 8.0, respectively.
Strain Gan-35 was inoculated into the screening medium containing 1.0, 2.0, and 3.5% (w/v) of NaCl, respectively, followed by incubation at 28 °C and 120 rpm for 48 h.
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