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RNAi-based phenotypic screening has demonstrated its utility in identifying cancer genes and putative drug targets [ 32- 34].
Population-level screening has demonstrated effectiveness with RCTs indicating a CRC mortality reduction between 15 to 33% [ 3, 4, 8- 10], with a slightly higher benefit secured from sigmoidoscopic screening [ 11].
On the other hand, radiographic screening has demonstrated benefits in screening large numbers of individuals in high prevalence populations where the background level of disease may be higher[ 12].
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Recent studies with array CGH screening have demonstrated a significant improvement in pregnancy outcomes for PGS patients [ 37, 64- 66].
Randomized controlled trials comparing VIA with no screening have demonstrated reductions in cervical cancer incidence and mortality through VIA-based screening., VIA is becoming a common approach to cervical cancer screening in Guatemala, a small Central American country with a large rural population.
These screens have demonstrated the power of forward genetic approaches for revealing functions of poorly annotated genes.
Recently, microarray screens have demonstrated increased IFN-inducible gene (IFIG) expression in peripheral blood mononuclear cells of patients with active SLE.
Large-scale RNAi screens have demonstrated that the function of a diverse population of genes with roles in many biological processes can be disrupted by the injection of double-stranded RNA (dsRNA) directly into the gonad [ 15], by soaking the nematodes in a dsRNA solution [ 16], or by feeding the nematodes bacteria expressing dsRNA [ 17, 18].
Mutagenesis studies and peptide library screens have demonstrated that Bcl-xL binds preferentially to peptides that include a phenylalanine or tyrosine to fill the enclosed hydrophobic pocket near 4a, whereas Mcl-1 tolerates a wide variety of substitutions at this position.
In unscreened populations alone, introduction of screening programs has demonstrated significant reductions in cervical cancer rates by 60%to90%0% within 3 years of screening instigation.
A real-time reverse transcriptase polymerase chain reaction (RT-PCR) screening study has demonstrated the specific overexpression of the HABP2 gene in lung adenocarcinoma, among six candidate marker genes for detection of non-small cell lung cancer [39].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com