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The washed cells were used for screening, growth assays, and ethanol fermentation as described below.
The spot culture growth inhibition (SPOTi) assay method has been enhanced in various studies using 6-well, 24-well and 48-well plates for screening growth inhibitors against mycobacteria.
In contrast, in two studies in centres without regular screening, growth and nutritional status deficits were more pronounced in children with T1DM + CD [ 16, 17] (Additional file 1: Table S1).
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KH performed siRNA screens, growth assays, cyclin B1 assays, apoptosis measurements, and prepared the manuscript.
To screen growth kinetics of S. bacillaris strain siva2011, algal biomass was harvested, and medium pH was measured after 1, 2, 3, 4, 5 and 6 days.
Using a commercial gene array for growth factors we screened growth factor production by IGF-I-treated astrocytes in response to H 2O 2. Among the several growth factors that increased, stem cell factor (SCF) showed the highest elevation (Table 3).
Enrichment map analysis using the Reactome Functional Interactome plugin in Cytoscape v2.8.1 [ 23, 24] of the genes ranked by primary screen growth inhibition showed four statistically significant linked networks and several singletons.
When screening for growth stocks, it's tempting to create rules requiring sky-high growth thresholds, such as earnings per share growth rates above 20 %, 30 or even 50%.
After systematic screening of growth conditions and host strains, TB medium was selected for expression of pEXP-hCRs.
Plant screening and growth.
Qualitative evaluation was performed by visual screening of growth on wort agar after spreading.
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