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This included 350 targets associated with the exons of genes (ESTs) and 2294 targets drawn from a library of BAC end sequences (BES) after screening for repeat sequences and location in the genome.
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In a study of patients admitted to emergency wards after a suicide attempt, the Suicide Assessment Scale SUASS, a 20-item scale) was found to perform well in screening for repeated suicide attempts in patients receiving ongoing psychiatric treatment but less well in screening for repeated suicide attempts in the entire study population [ 6].
All non-redundant trimmed sequences were screened for repeat motifs using the Repeatmasker (http://www.repeatmasker.org/), SSRIT (Simple Sequence Repeat Identification) http://www.gramene.org/db/markers/ssrtool using Perl regular expressions to find perfect SSR within the sequences (Temnykh et al. 2001).
288 identified SSR loci were screened for repeat variation in the eight animals listed above.
The gene structures of these genes were screened for repeat elements using a P. glauca repeat library (see Methods).
Sequences were analyzed in Staden Package (Staden, 1996) and screened for repeat sequences using Imperfect SSR Finder (Stieneke and Eujayl, 2007).
All sequences were screened for repeat loci using MISA, AC/GT repeats accounted for 41.5%, GA/TC repeats accounted for 55.3%, which was in consistence with results acquired by different anchoring primers.
Following an unclear result in kit one, if both kits are normal then patients are returned to the screening programme for repeat testing in 2 years' time.
Subsequent to microarray fabrication, the consensus sequences were screened for repeats using a custom salmonid repeat database with RepeatMasker.
Alignments for these genes were downloaded from the author's website http://compgen.bscb.cornell.edu/projects/mammal-psg/lrtall.txt and screened for repeats.
Finally the number of genes that were screened for repeats in each species was 13,926 human, 11,120 chimpanzee, 13,921 mouse, 10,360 rat, 7,073 cow and 7,834 dog genes.
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