Your English writing platform
Discover LudwigExact(4)
DT40 lines stably expressing chicken CIB1 or human CIB1 were generated by electroporating linearized (BglII) p6xmyc-hCIB1 or p6xmyc-cCIB1 (250 V, 950 µF, 2 pulses; BioRad GenePulser II) into surface Ig negative DT40, selecting with 1.5 mg/ml Geneticin (Invitrogen), and screening for expression by immunoblotting.
We first performed a screening for expression of key activation and functional molecules.
Revertant cells were obtained by transfecting Id1-overexpressing cells with a circular plasmid encoding Cre recombinase and screening for expression of GFP.
In libraries made in the pG8SAET-vector, an increase in the number of correct clones can also be followed by screening for expression of the E-tag.
Similar(56)
Test infections were screened for expression levels of the target proteins by Western blot analysis, and the highest expressing virus stock was used.
iHeps were then screened for expression of several growth factors, receptors and transcription factors (TFs) critical to liver development using reverse transcription-polymerase chain reaction (RT-PCR).
Feces of the PCR positive offspring screened for expression of human CEACAM1.
An archive of colorectal cancer (CRC) and normal colon tissue was screened for expression of A-type lamins.
Colonies were screened for expression of DsRed and those with the strongest signal were then tested for activity in Y. pestis.
Stable clones were selected with use of puromycin (1 µg/ml); and after they were established, they were screened for expression levels of Chk2 protein relative to control.
In initial experiments, splenic follicular, marginal zone, peritoneal B-1 and peyer's patch B-cells were screened for expression of 9 known TLRs.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com