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These include screening candidate radiosensitivity genes for mutations (Leong et al, 2000; Severin et al, 2001), adopting functional genomics approaches (e.g. using DNA microarrays) or, as adopted here, screening for abnormalities in key candidate proteins.
Given the radiation-sensitive phenotype displayed by mutants defective in components of the NHEJ pathway, in multiple eukaryotic species, we reasoned that these proteins were good candidates for screening for abnormalities in radiation-sensitive cancer patients.
Taken together, the results of this study and the controversial literature quoted above suggest that screening for abnormalities of DNA repair proteins, hMre11, Rad50 and Rad51, using Western blotting is unlikely to be useful for predicting clinical radiosensitivity.
These results suggest that mutations that affect protein expression of these factors do not account for most cases of clinical radiation hypersensitivity, and that screening for abnormalities of these factors using Western blotting might be unlikely to be useful for predicting clinical response to RT.
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Dr. Peter A. Benn, a geneticist at the University of Connecticut Health Center in Farmington, where the study was done, said fewer women had the procedures because more of them had blood tests and sonograms to screen for abnormalities.
In addition to the growing sophistication of pregnancy testing -- amnio and chorionic villus sampling and sonograms and specialized blood screens -- some fertile couples now spend the money for in vitro fertilization, accompanied by genetic analysis before the embryo is implanted, to screen for abnormalities that may not kick in for 20 years.
In any case, at least the joint adjacent to the symptomatic area should be imaged in order to screen for abnormalities (Van den Wyngaert et al. 2016).
We examined 50 human primary breast tumours for stathmin mRNA and protein expression and screened for abnormalities in the chromosome region harbouring the stathmin gene.
In order to explain the observed developmental and physiological phenotypes linked to ciliary dysfunction, we screened for abnormalities in morphology of the ciliary shaft and basal bodies in morphant embryos by immunofluorescence microscopy.
Testicular ultrasound (which should be performed using a 7.5 MHz probe) is used in primary assessment of the testes to confirm diagnosis, to distinguish from other scrotal abnormalities and to screen for abnormalities such as microlithiasis in the contralateral testis [ 2, 4– 6].
Neuromuscular ultrasound probably adds value to electrodiagnostic studies when diagnosing CTS and should be considered in screening for structural abnormalities at the wrist in those with CTS (Level B).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com